重点：

摘要：

Neutrophil-derived microvesicles enter cartilage and protect the joint in inflammatory arthritis

Highlights:

1. Neutrophilsare recruited to the joint during the active flares of arthritis and release high number of MVs

2. MVs can enter cartilage and bine the FRP2/ALX receptor, inducing TGF-B1 productionand ECM deposition, while protecting chondrocytes from apoptosis.

3. MVs carry a complex cargo that includes that tissue-protective, proresolving proteinAnxA1.

Summary:

Microvesicles(MVs) are emerging as a new mechanism of intercellular communication bytransferring cellular lipid and protein components to target cells, yet their function in disease is only now being explored. Headland et al. demonstrated a higher concentration of neutrophil-derived MVs in synovial fluid from rheumatoid arthritis patients. In addition, they also found that synovial MVs overexpressed the anti-inflammatory protein annexin A1 (AnxA1). TMEM-deficient mice (TMEM required for microvesiculation) exhibited intensified cartilage damage when subjected to inflammatory arthritis. To further study the function MVs, they tested the role of MVs in rodent models and in human primary chondrocytes; results showed that exogenous MVs were able to activate an abolicgene expression in chondrocytes, leading to extracellular matrix accumulationand cartilage protection by reducing interleukin-8 and prostaglandin E2.Results in vivowere alsoshown to be beneficial, reducing cartilage degradation cause by inflammatory arthritis. Mechanistic studies revealed that MVs could penetrate the cartilage,and that MVs-associated Anxn1 interacted with its receptor FRP2 and increasedTFG-b production, leading to cartilage protection. This unique therapeutic strategy could be utilized for protection in disease associated with cartilage degeneration.

二次整理者： 阮登峰/沈炜亮

浙江大学医学院附属第二医院·骨科；

浙江大学骨科研究所；

浙江大学李达三·叶耀珍再生医学发展基金；

浙江省组织工程与再生医学技术重点实验室；

中国医师协会骨科医师分会再生医学工作组；

本人专业诊治范围：

1，肌腱病&肌腱/韧带损伤：肘-网球肘；肩-肩周炎，肩袖损伤；膝-弹跳膝，跑步者膝，前交叉韧带损伤；踝-跟腱炎，跟腱断裂；手腕部-腱鞘炎；筋膜炎

2，再生医学技术治疗运动系统疑难杂症，包括：软骨/半月板/肌腱/韧带的修复、重建和再生；

门诊类型：肌腱病专科门诊！关节外科/运动医学专家门诊；

致谢：感谢 浙江省组织工程与再生医学技术重点实验室 kim 的分享！